These are worst-case conditions as in the permeability experiments, the exposure lasted 3 h and was executed on fully differentiated cells. For an initial feasibility experiment, we selected the compound granisetron (ESM Fig. J Pharmacol Exp Ther. Monitoring cell secretions on microfluidic chips using solid-phase extraction with mass spectrometry. Non-targeted and targeted analysis of collagen hydrolysates during the course...Non-targeted and targeted analysis of collagen hydrolysates during the course of digestion and absorptionIR action spectroscopy of glycosaminoglycan oligosaccharidesIR action spectroscopy of glycosaminoglycan oligosaccharidesBioanalytical method validation: How much should we do and how should we...Bioanalytical method validation: How much should we do and how should we document?Development of a triculture based system for improved benefit/risk assessment...Development of a triculture based system for improved benefit/risk assessment in pharmacology and human foodTransport Inhibition of Digoxin Using Several Common P-gp Expressing Cell...Transport Inhibition of Digoxin Using Several Common P-gp Expressing Cell Lines Is Not Necessarily Reporting Only on Inhibitor Binding to P-gpPermeation of Insulin, Calcitonin and Exenatide across Caco-2 Monolayers...Permeation of Insulin, Calcitonin and Exenatide across Caco-2 Monolayers: Measurement Using a Rapid, 3-Day SystemTitanium dioxide nanoparticle impact and translocation through ex vivo, in...Titanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epitheliaIntegrated Gut and Liver Microphysiological Systems for Quantitative In Vitro...Integrated Gut and Liver Microphysiological Systems for Quantitative In Vitro Pharmacokinetic StudiesRobust bioengineered 3D functional human intestinal epitheliumRobust bioengineered 3D functional human intestinal epitheliumEffects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires...Effects of differently shaped TiO2NPs (nanospheres, nanorods and nanowires) on the in vitro model (Caco-2/HT29) of the intestinal barrier 2013;34(6):321–35.PubMedCrossRefPubMedCentralGoogle Scholar The switching valves allowed for alternating UPLC-QTOF-MS measurements of the apical and basolateral sides of the flow-through Transwell system. Int J Pharm. The concentration of verapamil in the unknown samples was then calculated using peak area measurements in the reconstructed ion current (RIC) of the respective MRM transitions via interpolation of the external matrix-matched calibration curve (r2 = 0.99). 50. 12.
Several different geometries and designs have been developed for gut-on-a-chip devices, but two main types of designs are commonly found in the literature. Analyst. Subsequently, WST-1 reagent (in cell culture medium without phenol red) was added to the cells (1:10, 100 μL/well).
However, these configurations experienced severe analyte carryover between the two effluent streams, due to shared capillary tubing (carryover experiments described in the ESM). Chirality is an interesting topic and it is meaningful to explore the interactions between chiral small molecules and stereoselective biomacromolecules, with pre-clinical and clinical significances. Implementation of a dynamic intestinal gut-on-a-chip barrier model for transport studies of lipophilic dioxin congeners. The biggest strength of the online analysis system is the combination of a functional dynamic biological model of the intestine with semi-continuous online analytical detection. The chromatograms shown in Fig. 1996;678(2):227–36.CrossRefGoogle Scholar Cell permeability experiments using static and dynamic offline Transwell systems Cells were stained for tight junction protein ZO-1/TJP1 (red) and cell nuclei (blue). 2b). Protopanaxadiol-type ginsenosides are main effective ingredients in ginseng and are prone to biotransformation into a pair of ocotillol C20-24 epoxide epimers, namely, (20S,24S)-epoxy … Acetonitrile and methanol were purchased from Actu-All Chemicals (Oss, The Netherlands). Introduction [28]. Transwell systems are inserts containing a porous membrane on which monolayers of cells can be grown, creating an apical (lumen) and basolateral (bloodstream) compartment. (a) Permeability of verapamil across a monolayer of Caco-2/HT29-MTX cells in a static Transwell system. 2017;3(6):951–9.CrossRefGoogle Scholar The gradient started at 0% B and, after 4 min, was linearly increased to 100% B in 4 min. Caco-2/HT29-MTX (dynamic) 2019 Feb 3;2019:4016323. doi: 10.1155/2019/4016323.